Molecular weight (DA)
40kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPS35. AA range:211-260
Specificity
MRP-S35 Polyclonal Antibody detects endogenous levels of MRP-S35 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on
Function
subunit:Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.,
Protein name
28S ribosomal protein S35 mitochondrial
Other name
MRPS35; MRPS28; HDCMD11P; MDS023; PSEC0213; 28S ribosomal protein S35; mitochondrial; MRP-S35; S35mt; 28S ribosomal protein S28, mitochondrial; MRP-S28; S28mt
Human protein sequence Database
P82673
Mouse protein sequence database
Q8BJZ4
Rat protein sequence database
Cellular localization
Mitochondrion .
Tissue expression
Colon,Dendritic cell,Embryo,Hematopoietic stem cell,Lung,Melanoma,P
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