Service process 

Byabscience has accumulated a lot of experience through continuous experimental optimization and improvement, and has a professional enzyme-linked immunosorbent research and development team. The ELISA kit independently developed using professional enzyme-linked immunosorbent technology can quantitatively detect antigens and qualitatively detect specific antibodies in serum and other samples. High-quality reagents, advanced instruments and correct operation are necessary conditions to ensure accurate and reliable ELISA test results. ELISA testing has great advantages in terms of convenience, stability, repeatability and reliability.

ELISA testing technology service content:

1. Double antibody sandwich method for antigen detection 2. Indirect method for antibody detection 3. Provide customers with various ELISA technologies for sample testing.

Notes:

I. Service Commitment:

Whenever you purchase any ELISA kit in our catalog, you only need to inform our salesperson of the type of animal (Human, Rat, Mouse, Rabbit, Monkey, Pig...) to be tested, the test indicators (interleukins, hormones) and the number of specimens (48T/96T). The test report of the spot product will be delivered to the customer within two weeks from the day the customer's specimen is received!

All scientific research units are welcome to carry out close cooperation with our company at different levels on various projects, seek win-win development, make progress together, and accumulate experience for the development of China's testing industry.

II. Sample Requirements

Before collecting specimens, there must be a complete plan, and it must be clear whether the components to be tested are stable enough. We advocate that fresh specimens be tested as soon as possible. For specimens tested on the day of collection, they should be stored at 4℃ for standby use. If there are special reasons for periodic specimen collection, please divide the specimens in time after modeling and store them at -20℃ or -70℃. Because there is a certain temperature difference between the ice room and room temperature, proteins are very easy to degrade, which directly affects the quality of the experiment, so avoid repeated freezing and thawing. Customers who are testing radioimmunoassay specimens on behalf of us must ask our sales staff for instructions before taking the specimens. Please communicate with our technical staff for specific operating precautions.

Liquid specimens: The specimens must be liquid and do not contain precipitation. Including serum, plasma, urine, pleural effusion, cerebrospinal fluid, cell culture supernatant, tissue homogenate, etc.

Serum: After the room temperature blood coagulates naturally for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant. If a precipitate is formed, centrifuge again.

Plasma: EDTA, sodium citrate or heparin should be selected as the anticoagulant according to the requirements of the kit. Add 10% (v/v) anticoagulant (0.1M sodium citrate or 1% heparin or 2.0% EDTA.Na2) and mix for 10-20 minutes, then centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate is formed, centrifuge again.

Urine, pleural effusion, cerebrospinal fluid: Collect with sterile tubes. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate is formed, centrifuge again.

Cell culture supernatant: When detecting secretory components, collect with sterile tubes. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. When detecting intracellular components, dilute the cell suspension with PBS (PH7.0-7.4) to a cell concentration of about 1 million/ml. Repeated freezing and thawing to destroy the cells and release the intracellular components. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate is formed during storage, centrifuge again.

Tissue specimens: After cutting the specimen, weigh it. Add a certain amount of PBS. 1μg/L protease inhibitor or 50U/ml Aprotinin can be added to the buffer. Homogenize the specimen by hand or with a homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Carefully collect the supernatant and store it at -20 degrees or -70 degrees. If necessary, the sample can be concentrated and dried. After packaging, one portion is ready for testing, and the rest is frozen for later use.

III. When sending the specimen, the following information must be indicated:

1. Specimen number; 2. Items tested; 3. Whether to do duplicate wells; 3. Contact information; 4. Whether the specimen is sent back after the experiment.

Customer Notice:

The customer shall be responsible for the materials and information provided. If the materials and information provided by the customer are inaccurate, the customer shall bear the experimental delay or economic loss caused by the inaccurate materials and information provided by the customer.

Service characteristic 

Service content 

Customer provided 

Delivery content