Molecular weight (DA)
89kD
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human MCM3. AA range:641-690
Specificity
MCM3 Polyclonal Antibody detects endogenous levels of MCM3 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012],
Function
function:Acts as a factor that allows the DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation.,miscellaneous:Associated with the replication-specific DNA polymerase alpha.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,
Protein name
DNA replication licensing factor MCM3
Other name
MCM3; DNA replication licensing factor MCM3; DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102
Fields
>>DNA replication;>>Cell cycle
Human protein sequence Database
P25205
Mouse protein sequence database
P25206
Rat protein sequence database
Cellular localization
Nucleus . Chromosome . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. .
Tissue expression
Cervix,Coronary artery,Epithelium,Lymph,Osteosarcoma,Pancre
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