Molecular weight (DA)
169kD
Immunogen
The antiserum was produced against synthesized peptide derived from human CLASP1. AA range:1171-1220
Specificity
CLASP1 Polyclonal Antibody detects endogenous levels of CLASP1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
cytoplasmic linker associated protein 1(CLASP1) Homo sapiens CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM, Mar 2008],
Function
function:Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the CLASP family.,similarity:Contains 7 HEAT repeats.,subcellular location:Localizes to microtubule plus ends. Localizes to centrosomes, kinetochor
Protein name
CLIP-associating protein 1
Other name
CLASP1; KIAA0622; MAST1; CLIP-associating protein 1; Cytoplasmic linker-associated protein 1; Multiple asters homolog 1; Protein Orbit homolog 1; hOrbit1
Human protein sequence Database
Q7Z460
Mouse protein sequence database
Q80TV8
Rat protein sequence database
Cellular localization
Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Golgi apparatus, trans-Golgi network . Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2.
Tissue expression
Brain,Endometrium carcinoma cell line,Epithelium,Eye,Platelet,Poole
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车