Molecular weight (DA)
50kD
Immunogen
The antiserum was produced against synthesized peptide derived from human p55CDC. AA range:81-130
Specificity
Cdc20 Polyclonal Antibody detects endogenous levels of Cdc20 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008],
Function
developmental stage:Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.,function:Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated during mitosis, probably by maturation promoting factor (MPF).,PTM:Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint.,similarity:Belongs to the WD repeat CDC20/Fizzy family.,similarity:Contains 7 WD repeats.,subunit:Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1 (By similarity). Interacts with MAD2L
Protein name
Cell division cycle protein 20 homolog
Other name
CDC20; Cell division cycle protein 20 homolog; p55CDC
Fields
>>Cell cycle;>>Oocyte meiosis;>>Ubiquitin mediated proteolysis;>>Human T-cell leukemia virus 1 infection;>>Viral carcinogenesis
Human protein sequence Database
Q12834
Mouse protein sequence database
Q9JJ66
Rat protein sequence database
Q62623
Cellular localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole .
Tissue expression
Colon,Colon adenocarcinoma,Liver,Lymph,Muscle,Ovary,Skin,Spleen,Testis,
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