Molecular weight (DA)
32kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Cyclin D3 around the phosphorylation site of Thr283. AA range:243-292
Specificity
Phospho-Cyclin D3 (T283) Polyclonal Antibody detects endogenous levels of Cyclin D3 protein only when phosphorylated at T283.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/5000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008],
Function
function:Essential for the control of the cell cycle at the G1/S (start) transition. Potentiates the transcriptional activity of ATF5.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin D subfamily.,subunit:Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with ATF5. Interacts with EIF3K.,
Protein name
G1/S-specific cyclin-D3
Other name
CCND3; G1/S-specific cyclin-D3
Fields
>>Cell cycle;>>p53 signaling pathway;>>PI3K-Akt signaling pathway;>>Cellular senescence;>>Wnt signaling pathway;>>Hippo signaling pathway;>>Focal adhesion;>>JAK-STAT signaling pathway;>>Measles;>>Influenza A;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Viral carcinogenesis;>>Chemical carcinogenesis - receptor activation
Human protein sequence Database
P30281
Mouse protein sequence database
P30282
Rat protein sequence database
P48961
Cellular localization
Nucleus . Cytoplasm .
Tissue expression
Brain,Lung,Placenta,Synovial membrane tissue,Umbilical cord blood,
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