Molecular weight (DA)
41kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MKP-1/2. AA range:261-310
Specificity
MKP-1/2 Polyclonal Antibody detects endogenous levels of MKP-1/2 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferati
Function
catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,function:Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.,induction:By oxidative stress and heat shock.,similarity:Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.,similarity:Contains 1 rhodanese domain.,similarity:Contains 1 tyrosine-protein phosphatase domain.,tissue specificity:Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.,
Protein name
Dual specificity protein phosphatase 1/4
Other name
DUSP1; CL100; MKP1; PTPN10; VH1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase CL100; DUSP4;
Fields
>>MAPK signaling pathway;>>Serotonergic synapse;>>Parkinson disease;>>Fluid shear stress and atherosclerosis
Human protein sequence Database
P28562/Q13115
Mouse gene ID
19252/319520
Mouse protein sequence database
Rat protein sequence database
Q64623/Q62767
Cellular localization
Nucleus .
Tissue expression
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
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