Molecular weight (DA)
37kD
Immunogen
Synthesized peptide derived from human DFFB
Specificity
This antibody detects endogenous levels of human DFFB
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC-p 1:50-200, WB 1:500-2000. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Background
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013],
Function
enzyme regulation:Inhibited by DFFA (DFF45).,function:Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.,similarity:Contains 1 CIDE-N domain.,subunit:Heterodimer of DFFA and DFFB.,
Gene Name
DFFB CAD DFF2 DFF40
Other name
DNA fragmentation factor subunit beta (EC 3.-.-.-;Caspase-activated deoxyribonuclease;CAD;Caspase-activated DNase;Caspase-activated nuclease;CPAN;DNA fragmentation factor 40 kDa subunit;DFF-40)
Human protein sequence Database
O76075
Mouse protein sequence database
Rat protein sequence database
Cellular localization
Cytoplasm. Nucleus.
Tissue expression
Brain,Fetal brain,Lung,Pancreas,
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车