Molecular weight (DA)
150-240kD
Immunogen
Synthesized peptide derived from human ALK (Phospho-Tyr1586)
Specificity
This antibody detects endogenous levels of ALK at Human:Y1586; Mouse:Y1592, It doesn't reacte with total protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC-p 1:50-200, WB 1:500-2000. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Background
This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome
Function
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.,disease:A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.,disease:A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.,function:Orphan receptor with a tyrosine-protein kinase activity. Appears to play an important role in the normal development and function
Protein name
ALK (Phospho-Tyr1586)
Other name
ALK tyrosine kinase receptor (EC 2.7.10.1;Anaplastic lymphoma kinase;CD antigen CD246)
Fields
>>Pathways in cancer;>>Non-small cell lung cancer;>>PD-L1 expression and PD-1 checkpoint pathway in cancer
Human protein sequence Database
Q9UM73
Mouse protein sequence database
Rat protein sequence database
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Membrane attachment is essential for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway. .
Tissue expression
Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
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