Molecular weight (DA)
50kD
Immunogen
Synthesized acetyl-peptide from human protein at AA range: 130-200
Specificity
This antibody detects endogenous levels of E2F-3 at Human:K168;Mouse:K160;Rat:K161, It doesn't reacte with total protein.
Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Dilution rate
WB: 1:500-10000 ELISA: 1:10000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Background
This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013],
Function
function:Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-3 binds specifically to RB1 protein, in a cell-cycle dependent manner.,similarity:Belongs to the E2F/DP family.,subunit:Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP.,
Protein name
E2F transcription factor 3
Fields
>>Endocrine resistance;>>Cell cycle;>>Cellular senescence;>>Cushing syndrome;>>Hepatitis C;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Pancreatic cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Bladder cancer;>>Chronic myeloid leukemia;>>Small cell lung cancer;>>Non-small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer
Human protein sequence Database
O00716
Mouse protein sequence database
O35261
Rat protein sequence database
Cellular localization
Nucleus.
Tissue expression
Bone marrow,PCR rescued clones,Placenta,Pre-B cell,Rectum tumor,Uterus,
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