Molecular weight (DA)
21kD
Immunogen
Synthesized peptide derived from MRP-L35 . at AA range: 80-160
Specificity
MRP-L35 Polyclonal Antibody detects endogenous levels of MRP-L35 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000;IHC-p 1:50-300
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p, 10q, and Xp. [provided by RefSeq, Jul 2008],
Function
similarity:Belongs to the ribosomal protein L35P family.,
Protein name
39S ribosomal protein L35 mitochondrial
Other name
MRPL35; BM-007; 39S ribosomal protein L35, mitochondrial; L35mt; MRP-L35
Human protein sequence Database
Q9NZE8
Mouse protein sequence database
Q9CQL6
Rat protein sequence database
Cellular localization
Mitochondrion .
Tissue expression
Bone marrow,Liver,Stomach,
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