Molecular weight (DA)
34kD
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human SPARC. AA range:141-190
Specificity
SPARC Polyclonal Antibody detects endogenous levels of SPARC protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015],
Function
developmental stage:Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.,function:Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.,online information:Osteonectin entry,similarity:Belongs to the SPARC family.,similarity:Contains 1 EF-hand domain.,similarity:Contains 1 follistatin-like domain.,similarity:Contains 1 Kazal-like domain.,subcellular location:In or around the basement membrane.,
Other name
SPARC; ON; SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
Human protein sequence Database
P09486
Mouse protein sequence database
P07214
Rat protein sequence database
P16975
Cellular localization
Secreted, extracellular space, extracellular matrix, basement membrane . In or around the basement membrane.
Tissue expression
Muscle,Placenta,PNS,Prostate,Skin,
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