Immunogen
The antiserum was produced against synthesized peptide derived from human URB1. AA range:2013-2062
Specificity
NPA1 Polyclonal Antibody detects endogenous levels of NPA1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
URB1, also named as C21orf108, KIAA0539 and NPA1, is a 2271 amino acid protein that is homologous to the S. cerevisiae protein URB1 which is a nucleolar protein involved in the normal accumulation of 25S and 5. S rRNAs and may also play a role in the biosynthesis of the 60S ribosomal unit. Also localized to the nucleolus, human URB1 is thought to have the same functions as S. cerevisiae URB1. The antibody is specific to URB1.
Protein name
Nucleolar pre-ribosomal-associated protein 1
Other name
URB1; C21orf108; KIAA0539; NOP254; NPA1; Nucleolar pre-ribosomal-associated protein 1; Nucleolar protein 254 kDa; URB1 ribosome biogenesis 1 homolog
Human protein sequence Database
O60287
Mouse protein sequence database
Q571H0
Rat protein sequence database
Cellular localization
Nucleus, nucleolus .
Tissue expression
Brain,Epithelium,Placenta,T-cell,
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