Immunogen
The antiserum was produced against synthesized peptide derived from human MRPL33. AA range:10-59
Specificity
MRP-L33 Polyclonal Antibody detects endogenous levels of MRP-L33 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
Function
caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,similarity:Belongs to the ribosomal protein L33P family.,
Protein name
39S ribosomal protein L33 mitochondrial
Other name
MRPL33; C2orf1; 39S ribosomal protein L33; mitochondrial; L33mt; MRP-L33
Human protein sequence Database
O75394
Mouse protein sequence database
Q9CQP0
Rat protein sequence database
Cellular localization
Mitochondrion .
Tissue expression
Brain,Pituitary,Testis,Umbilical cord blood,