Molecular weight (DA)
215kD
Immunogen
The antiserum was produced against synthesized peptide derived from human DOCK1. AA range:1661-1710
Specificity
DOCK 180 Polyclonal Antibody detects endogenous levels of DOCK 180 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014],
Function
domain:The DHR-2 domain is necessary and sufficient for the GEF activity.,function:Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.,similarity:Belongs to the DOCK family.,similarity:Contains 1 DHR-1 (CZH-1) domain.,similarity:Contains 1 DHR-2 (CZH-2) domain.,similarity:Contains 1 SH3 domain.,subcellular location:Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.,subunit:Interacts with the SH3 domains of CRK and NCK2 via multiple sites. Interacts with nucleotide-free RAC1 via its DHR-2 domain. Interacts with ELMO1, ELMO2 and probably ELMO3 via its SH3 domain. Interacts with RAC1 and BAI1.,tissue specificity:Highly expressed
Protein name
Dedicator of cytokinesis protein 1
Other name
DOCK1; Dedicator of cytokinesis protein 1; 180 kDa protein downstream of CRK; DOCK180
Fields
>>Focal adhesion;>>Fc gamma R-mediated phagocytosis;>>Regulation of actin cytoskeleton;>>Bacterial invasion of epithelial cells;>>Shigellosis;>>Yersinia infection
Human protein sequence Database
Q14185
Mouse protein sequence database
Q8BUR4
Rat protein sequence database
Cellular localization
Cytoplasm . Membrane . Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-trisphosphate. .
Tissue expression
Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车