Molecular weight (DA)
80kD
Immunogen
The antiserum was produced against synthesized peptide derived from human eIF4B around the phosphorylation site of Ser422. AA range:388-437
Specificity
Phospho-eIF4B (S422) Polyclonal Antibody detects endogenous levels of eIF4B protein only when phosphorylated at S422.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
function:Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Self-associates and interacts with EIF3 p170 subunit.,
Function
function:Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Self-associates and interacts with EIF3 p170 subunit.,
Protein name
Eukaryotic translation initiation factor 4B
Other name
EIF4B; Eukaryotic translation initiation factor 4B; eIF-4B
Fields
>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Proteoglycans in cancer
Human protein sequence Database
P23588
Mouse protein sequence database
Q8BGD9
Rat protein sequence database
Cellular localization
cytosol,polysome,eukaryotic translation initiation factor 4F complex,dendrite,neuronal cell body,
Tissue expression
Adipose tissue,Brain,Cervix carcinoma,Epithelium,Liver,Lung
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