Molecular weight (DA)
120kD
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human DPYD. AA range:351-400
Specificity
DPYD Polyclonal Antibody detects endogenous levels of DPYD protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
Function
catalytic activity:5,6-dihydrouracil + NADP(+) = uracil + NADPH.,cofactor:Binds 2 4Fe-4S clusters. Contains approximately 33 iron atoms per molecule.,cofactor:Binds 2 FAD.,cofactor:Binds 2 FMN.,disease:Defects in DPYD are the cause of dihydropyrimidine dehydrogenase deficiency (DPYD deficiency) [MIM:274270]; also known as hereditary thymine-uraciluria or familial pyrimidinemia. DPYD deficiency is a disease characterized by persistent urinary excretion of excessive amounts of uracil, thymine and 5-hydroxymethyluracil. Patients suffering from this disease show a severe reaction to the anticancer drug 5-fluorouracil. This reaction includes stomatitis, Leukopenia, thrombocytopenia, hair loss, diarrhea, fever, marked weight loss, cerebellar ataxia, and neurologic symptoms, progressing to semicoma.,function:Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine.
Protein name
Dihydropyrimidine dehydrogenase [NADP(+)]
Other name
DPYD; Dihydropyrimidine dehydrogenase [NADP(+)]; DHPDHase; DPD; Dihydrothymine dehydrogenase; Dihydrouracil dehydrogenase
Fields
>>Pyrimidine metabolism;>>beta-Alanine metabolism;>>Pantothenate and CoA biosynthesis;>>Drug metabolism - other enzymes;>>Metabolic pathways
Human protein sequence Database
Q12882
Mouse protein sequence database
Q8CHR6
Rat protein sequence database
O89000
Cellular localization
Cytoplasm.
Tissue expression
Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells.
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