Molecular weight (DA)
35kD
Immunogen
The antiserum was produced against synthesized peptide derived from human CA5A. AA range:171-220
Specificity
CA VA Polyclonal Antibody detects endogenous levels of CA VA protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VA is localized in the mitochondria and expressed primarily in the liver. It may play an important role in ureagenesis and gluconeogenesis. CA5A gene maps to chromosome 16q24.3 and an unprocessed pseudogene has been assigned to 16p12-p11.2. [provided by RefSeq, Jul 2008],
Function
catalytic activity:H(2)CO(3) = CO(2) + H(2)O.,cofactor:Zinc.,function:Reversible hydration of carbon dioxide.,similarity:Belongs to the alpha-carbonic anhydrase family.,
Protein name
Carbonic anhydrase 5A mitochondrial
Other name
CA5A; CA5; Carbonic anhydrase 5A; mitochondrial; Carbonate dehydratase VA; Carbonic anhydrase VA; CA-VA
Fields
>>Nitrogen metabolism;>>Metabolic pathways
Human protein sequence Database
P35218
Mouse protein sequence database
P23589
Rat protein sequence database
Cellular localization
Mitochondrion .
Tissue expression
Liver,Umbilical cord,
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