Molecular weight (DA)
35kD
Immunogen
The antiserum was produced against synthesized peptide derived from human PHCA. AA range:171-220
Specificity
APHC Polyclonal Antibody detects endogenous levels of APHC protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
enzyme regulation:Activated by Ca(2+) and inhibited by Zn(2+).,function:Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.,similarity:Belongs to the alkaline ceramidase family.,tissue specificity:Ubiquitously expressed. Highest expression in placenta.,
Function
enzyme regulation:Activated by Ca(2+) and inhibited by Zn(2+).,function:Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.,similarity:Belongs to the alkaline ceramidase family.,tissue specificity:Ubiquitously expressed. Highest expression in placenta.,
Protein name
Alkaline ceramidase 3
Other name
ACER3; APHC; PHCA; Alkaline ceramidase 3; AlkCDase 3; Alkaline CDase 3; Alkaline dihydroceramidase SB89; Alkaline phytoceramidase; aPHC
Fields
>>Sphingolipid metabolism;>>Metabolic pathways
Human protein sequence Database
Q9NUN7
Mouse protein sequence database
Q9D099
Rat protein sequence database
Cellular localization
Endoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein .
Tissue expression
Ubiquitously expressed. Highly expressed in placenta (PubMed:11356846). Expressed in erythrocytes (PubMed:20207939).
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