Molecular weight (DA)
33kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MBD3. AA range:221-270
Specificity
MBD3 Polyclonal Antibody detects endogenous levels of MBD3 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013],
Function
function:Does not bind DNA by itself. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing.,similarity:Contains 1 MBD (methyl-CpG-binding) domain.,subcellular location:Nuclear, in discrete foci.,subunit:Heterodimer with MBD2. Part of the NuRD and the MeCP1 complex. Binds HDAC1, MTA2, DNMT1, p66-alpha and p66-beta.,
Protein name
Methyl-CpG-binding domain protein 3
Other name
MBD3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3
Human protein sequence Database
O95983
Mouse protein sequence database
Q9Z2D8
Rat protein sequence database
Cellular localization
Nucleus. Chromosome. Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes.
Tissue expression
Brain,Cervix,Eye,Muscle,Uterus,
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