Molecular weight (DA)
60kD
Immunogen
The antiserum was produced against synthesized peptide derived from human HARS. AA range:460-509
Specificity
HisRS Polyclonal Antibody detects endogenous levels of HisRS protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012],
Function
catalytic activity:ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).,similarity:Belongs to the class-II aminoacyl-tRNA synthetase family.,similarity:Contains 1 WHEP-TRS domain.,tissue specificity:Brain, heart, liver and kidney.,
Protein name
Histidine--tRNA ligase cytoplasmic
Other name
HARS; HRS; Histidine--tRNA ligase; cytoplasmic; Histidyl-tRNA synthetase; HisRS
Fields
>>Aminoacyl-tRNA biosynthesis
Human protein sequence Database
P12081
Mouse protein sequence database
Q61035
Rat protein sequence database
Cellular localization
Cytoplasm .
Tissue expression
Brain, heart, liver and kidney.
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车