Molecular weight (DA)
38kD
Immunogen
The antiserum was produced against synthesized peptide derived from human DNA Polymerase beta. AA range:286-335
Specificity
DNA pol β Polyclonal Antibody detects endogenous levels of DNA pol β protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011],
Function
catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Binds 2 magnesium ions per subunit.,domain:Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.,function:Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.,PTM:Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.,similarity:Belongs to the DNA polymerase type-X family.,subunit:Monomer.,
Protein name
DNA polymerase beta
Other name
POLB; DNA polymerase beta
Fields
>>Base excision repair;>>Human T-cell leukemia virus 1 infection;>>Viral carcinogenesis
Human protein sequence Database
P06746
Mouse protein sequence database
Q8K409
Rat protein sequence database
P06766
Cellular localization
Nucleus. Cytoplasm. Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.
Tissue expression
Skin,Testis,
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