Molecular weight (DA)
38kD
Immunogen
The antiserum was produced against synthesized peptide derived from human IkappaB-epsilon around the phosphorylation site of Ser22. AA range:131-180
Specificity
Phospho-IκB-ε (S22) Polyclonal Antibody detects endogenous levels of IκB-ε protein only when phosphorylated at S22.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011],
Function
function:Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.,PTM:Serine phosphorylated; followed by proteasome-dependent degradation.,similarity:Belongs to the NF-kappa-B inhibitor family.,similarity:Contains 6 ANK repeats.,subunit:Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit.,tissue specificity:Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.,
Protein name
NF-kappa-B inhibitor epsilon
Other name
NFKBIE; IKBE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; I-kappa-B-epsilon; IkB-E; IkB-epsilon; IkappaBepsilon
Fields
>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>B cell receptor signaling pathway;>>Neurotrophin signaling pathway;>>Adipocytokine signaling pathway;>>Epstein-Barr virus infection;>>PD-L1 expression and PD-1 checkpoint pathway in cancer
Human protein sequence Database
O00221
Mouse protein sequence database
O54910
Rat protein sequence database
Cellular localization
Cytoplasm .
Tissue expression
Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
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