Molecular weight (DA)
51kD
Immunogen
The antiserum was produced against synthesized peptide derived from human hnRNP K around the phosphorylation site of Ser284. AA range:250-299
Specificity
Phospho-hnRNP K (S284) Polyclonal Antibody detects endogenous levels of hnRNP K protein only when phosphorylated at S284.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have
Function
function:One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.,mass spectrometry: PubMed:11840567,PTM:Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.,similarity:Contains 1 KH domain.,similarity:Contains 2 KH domains.,similarity:Contains 3 KH domains.,subcellular location:In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.,subunit:Interacts with RBM42 and ZIK1 (By similarity). Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRNPK, HNR
Protein name
Heterogeneous nuclear ribonucleoprotein K
Other name
HNRNPK; HNRPK; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; Transformation up-regulated nuclear protein; TUNP
Fields
>>Spliceosome;>>Viral carcinogenesis;>>MicroRNAs in cancer
Human protein sequence Database
P61978
Mouse protein sequence database
P61979
Rat protein sequence database
P61980
Cellular localization
Cytoplasm . Nucleus, nucleoplasm . Cell projection, podosome . Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702).
Tissue expression
Brain,Cajal-Retzius cell,Colorectal cancer and surrounding
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