Molecular weight (DA)
47kD
Immunogen
The antiserum was produced against synthesized peptide derived from human BLZF1. AA range:10-59
Specificity
Golgin 45 Polyclonal Antibody detects endogenous levels of Golgin 45 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
caution:Because of the presence of a potential basic motif and leucine-zipper domain, PubMed:9129147 and PubMed:11056056 have thought that BLZF1 is a potential transcription factor. They found it localized in the nucleus, except isoform 2, which was cytoplasmic. However, homology at several typical position for basic or hydrophobic residues is missing.,function:Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.,induction:Up-regulated by retinoids.,subunit:Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi.,tissue specificity:Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells.,
Function
caution:Because of the presence of a potential basic motif and leucine-zipper domain, PubMed:9129147 and PubMed:11056056 have thought that BLZF1 is a potential transcription factor. They found it localized in the nucleus, except isoform 2, which was cytoplasmic. However, homology at several typical position for basic or hydrophobic residues is missing.,function:Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.,induction:Up-regulated by retinoids.,subunit:Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi.,tissue specificity:Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines,
Other name
BLZF1; JEM1; Golgin-45; Basic leucine zipper nuclear factor 1; JEM-1; p45 basic leucine-zipper nuclear factor
Human protein sequence Database
Q9H2G9
Mouse protein sequence database
Q8R2X8
Rat protein sequence database
Cellular localization
Golgi apparatus membrane .; [Isoform 1]: Nucleus . Detected in the nucleus upon heterologous expression. Not detected in the cytoplasm. .; [Isoform 2]: Cytoplasm . Not detected in the nucleus. .
Tissue expression
Detected in adrenal gland (PubMed:9129147).
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