Molecular weight (DA)
40kD
Immunogen
The antiserum was produced against synthesized peptide derived from human ARFIP1. AA range:271-320
Specificity
Arfaptin 1 Polyclonal Antibody detects endogenous levels of Arfaptin 1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
function:Putative target protein of ADP-ribosylation factor.,similarity:Contains 1 AH domain.,subunit:Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6.,tissue specificity:Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart.,
Function
function:Putative target protein of ADP-ribosylation factor.,similarity:Contains 1 AH domain.,subunit:Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6.,tissue specificity:Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart.,
Other name
ARFIP1; Arfaptin-1; ADP-ribosylation factor-interacting protein 1
Human protein sequence Database
P53367
Mouse protein sequence database
Rat protein sequence database
Q9JHU5
Cellular localization
Golgi apparatus . Golgi apparatus, trans-Golgi network membrane .
Tissue expression
Ubiquitously expressed (PubMed:9038142). Higher levels in liver, pancreas, placenta, skeletal muscle and heart (PubMed:9038142).
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