Immunogen
The antiserum was produced against synthesized peptide derived from human Collagen XII alpha1. AA range:1481-1530
Specificity
COL12A1 Polyclonal Antibody detects endogenous levels of COL12A1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes the alpha chain of type XII collagen, a member of the FACIT (fibril-associated collagens with interrupted triple helices) collagen family. Type XII collagen is a homotrimer found in association with type I collagen, an association that is thought to modify the interactions between collagen I fibrils and the surrounding matrix. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
Function
alternative products:The final tissue form of collagen XII may contain homotrimers of either isoform 1 or isoform 2 or any combination of isoform 1 and isoform 2,function:Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.,PTM:Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.,PTM:O-glycosylation of isoform 1; glycosaminoglycan of chondroitin-sulfate type.,PTM:The triple-helical tail is stabilized by disulfide bonds at each end.,similarity:Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.,similarity:Contains 1 TSP N-terminal (TSPN) domain.,similarity:Contains 18 fibronectin type-III dom
Protein name
Collagen alpha-1(XII) chain
Other name
COL12A1; COL12A1L; Collagen alpha-1(XII) chain
Fields
>>Protein digestion and absorption
Human protein sequence Database
Q99715
Mouse protein sequence database
Q60847
Rat protein sequence database
Cellular localization
Secreted, extracellular space, extracellular matrix .
Tissue expression
Found in collagen I-containing tissues: both isoform 1 and isoform 2 appear in amnion, chorion, skeletal muscle, small intestine, and in cell culture of dermal fibroblasts, keratinocytes and endothelial cells. Only isoform 2 is found in lung, placenta, kidney and a squamous cell carcinoma cell line. Isoform 1 is also present in the corneal epithelial Bowman's membrane (BM) and the interfibrillar matrix of the corneal stroma, but it is not detected in the limbal BM.
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