Molecular weight (DA)
36kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MAT1. AA range:91-140
Specificity
Mat1 Polyclonal Antibody detects endogenous levels of Mat1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
Function
function:Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 1 UIM (ubiquitin-interacting motif) repeat.,subunit:Associates primarily with CDK7 and cyclin H to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor.,tissue specificity:Highest levels in colon and testis. Moderate le
Protein name
CDK-activating kinase assembly factor MAT1
Other name
MNAT1; CAP35; MAT1; RNF66; CDK-activating kinase assembly factor MAT1; CDK7/cyclin-H assembly factor; Cyclin-G1-interacting protein; Menage a trois; RING finger protein 66; RING finger protein MAT1; p35; p36
Fields
>>Basal transcription factors;>>Nucleotide excision repair
Human protein sequence Database
P51948
Mouse protein sequence database
P51949
Rat protein sequence database
Cellular localization
Nucleus.
Tissue expression
Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.
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