Immunogen
The antiserum was produced against synthesized peptide derived from human Insulin. AA range:49-98
Specificity
Insulin Polyclonal Antibody detects endogenous levels of Insulin protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010],
Function
disease:Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].,function:Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.,function:Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.,function:The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.,mass spectrometry: PubMed:12586351; PubMed:15359740,online information:Clinical information on Eli Lilly insu
Fields
>>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>cGMP-PKG signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Phospholipase D signaling pathway;>>Oocyte meiosis;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Regulation of actin cytoskeleton;>>Insulin signaling pathway;>>Insulin secretion;>>Ovarian steroidogenesis;>>Progesterone-mediated oocyte maturation;>>Prolactin signaling pathway;>>Regulation of lipolysis in adipocytes;>>Type II diabetes mellitus;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Type I diabetes mellitus;>>Maturity onset diabetes of the young;>>Aldosterone-regulated sodium reabsorption;>>Alzheimer disease;>>Prostate cancer;>>Diabetic cardiomyopathy
Human protein sequence Database
P01308
Mouse protein sequence database
Rat protein sequence database
Cellular localization
Secreted.
Tissue expression
Blood,Liver,Muscle,Pancreas,
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