Molecular weight (DA)
23kD
Immunogen
The antiserum was produced against synthesized peptide derived from human NM23. AA range:91-140
Specificity
NM23-H2 Polyclonal Antibody detects endogenous levels of NM23-H2 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010],
Function
catalytic activity:ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.,cofactor:Magnesium.,disease:This protein is found in reduced amount in tumor cells of high metastatic potential.,disease:This protein is found in reduced amount in tumor cells of high metastatic potential. Somatic mutations of NME1 are found in neuroblastoma. Increased NME1 in neuroblastoma is correlated with features of the disease that are associated with aggressive tumors. May therefore have distinct if not opposite roles in different tumors.,enzyme regulation:Autophosphorylation at His-118 increases serine/threonine protein kinase activity of the enzyme. Interaction with the SET complex inhibits exonuclease activity.,function:Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the
Protein name
Nucleoside diphosphate kinase B
Other name
NME2; NM23B; Nucleoside diphosphate kinase B; NDK B; NDP kinase B; C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB; nm23-H2
Fields
>>Purine metabolism;>>Pyrimidine metabolism;>>Drug metabolism - other enzymes;>>Metabolic pathways;>>Nucleotide metabolism;>>Biosynthesis of cofactors
Human gene ID
4831/654364
Human protein sequence Database
P22392
Mouse protein sequence database
Q01768
Rat protein sequence database
P19804
Cellular localization
Cytoplasm . Cell projection, lamellipodium . Cell projection, ruffle . Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates. .; [Isoform 1]: Cytoplasm . Cytoplasm, perinuclear region . Nucleus .; [Isoform 3]: Cytoplasm . Cytoplasm, perinuclear region . Nucleus .
Tissue expression
[Isoform 1]: Ubiquitously expressed. ; [Isoform 3]: Ubiquitously expressed.
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