Immunogen
The antiserum was produced against synthesized peptide derived from human MYT1 around the phosphorylation site of Ser83. AA range:49-98
Specificity
Phospho-Myt 1 (S83) Polyclonal Antibody detects endogenous levels of Myt 1 protein only when phosphorylated at S83.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/5000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],
Function
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.,enzyme regulation:Negatively regulated by hyperphosphorylation during mitosis. The hyperphosphorylated form does not associate with CCNB1-CDC2 complexes. The PLK1 protein kinase may be required for mitotic phosphorylation.,function:Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the cdc2 kinase specifically when cdc2 is complexed to cyclins. Mediates phosphorylation of cdc2 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of cdc2 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be ind
Protein name
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
Other name
PKMYT1; MYT1; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; Myt1 kinase
Fields
>>Cell cycle;>>Oocyte meiosis;>>Progesterone-mediated oocyte maturation
Human protein sequence Database
Q99640
Mouse protein sequence database
Q9ESG9
Rat protein sequence database
Cellular localization
Endoplasmic reticulum membrane ; Peripheral membrane protein . Golgi apparatus membrane ; Peripheral membrane protein .
Tissue expression
Brain,Epithelium,PCR rescued clones,
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