Immunogen
The antiserum was produced against synthesized peptide derived from human PP1-alpha around the phosphorylation site of Thr320. AA range:281-330
Specificity
Phospho-PP1α (T320) Polyclonal Antibody detects endogenous levels of PP1α protein only when phosphorylated at T320.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function
catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,cofactor:Binds 1 iron ion per subunit.,cofactor:Binds 1 manganese ion per subunit.,enzyme regulation:The phosphatase activity of the PPP1R15A-PP1 complex toward EIF2S1 is specifically inhibited by Salubrinal, a drug that protects cells from endoplasmic reticulum stress.,function:Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II.,online information:The th
Protein name
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Other name
PPP1CA; PPP1A; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; PP-1A
Fields
>>mRNA surveillance pathway;>>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Oocyte meiosis;>>Cellular senescence;>>Adrenergic signaling in cardiomyocytes;>>Vascular smooth muscle contraction;>>Hippo signaling pathway;>>Focal adhesion;>>Platelet activation;>>Long-term potentiation;>>Dopaminergic synapse;>>Inflammatory mediator regulation of TRP channels;>>Regulation of actin cytoskeleton;>>Insulin signaling pathway;>>Oxytocin signaling pathway;>>Insulin resistance;>>Amphetamine addiction;>>Alcoholism;>>Herpes simplex virus 1 infection;>>Proteoglycans in cancer;>>Diabetic cardiomyopathy
Human protein sequence Database
P62136
Mouse protein sequence database
P62137
Rat protein sequence database
P62138
Cellular localization
Cytoplasm . Nucleus . Nucleus, nucleoplasm . Nucleus, nucleolus . Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles. Shuttles toward the cytosol during infection with VEEV (PubMed:29769351). .
Tissue expression
Colon carcinoma,Liver,Lung,Muscle,Pancreas,Placenta,Platele
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