Molecular weight (DA)
45kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Pim-1 around the phosphorylation site of Tyr309. AA range:281-330
Specificity
Phospho-Pim-1 (Y309) Polyclonal Antibody detects endogenous levels of Pim-1 protein only when phosphorylated at Y309.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011],
Function
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Manganese.,function:Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provide a selective advantage in tumorigenesis. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3.,induction:Strongly induced in leukocytes by the JAK/STAT pathway in response to cytokines.,PTM:Autophosphorylated on both serine/threonine and tyrosine residues.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Binds to RP9. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Isoform 1, but not isoform 2, binds BMX.,tissue specificity:Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 an
Protein name
Serine/threonine-protein kinase pim-1
Other name
PIM1; Serine/threonine-protein kinase pim-1
Fields
>>JAK-STAT signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Pathways in cancer;>>MicroRNAs in cancer;>>Acute myeloid leukemia
Human protein sequence Database
P11309
Mouse protein sequence database
P06803
Rat protein sequence database
P26794
Cellular localization
[Isoform 1]: Cytoplasm. Nucleus.; [Isoform 2]: Cell membrane.
Tissue expression
Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
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