Molecular weight (DA)
150kD
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human PTPRJ. AA range:861-910
Specificity
CD148 Polyclonal Antibody detects endogenous levels of CD148 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms
Function
catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,disease:Defects in PTPRJ are found in cancers of colon, lung, and breast.,function:May contribute to the mechanism of contact inhibition of cell growth.,PTM:N- and O-glycosylated.,similarity:Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.,similarity:Contains 1 tyrosine-protein phosphatase domain.,similarity:Contains 9 fibronectin type-III domains.,
Protein name
Receptor-type tyrosine-protein phosphatase eta
Other name
PTPRJ; DEP1; Receptor-type tyrosine-protein phosphatase eta; Protein-tyrosine phosphatase eta; R-PTP-eta; Density-enhanced phosphatase 1; DEP-1; HPTP eta; Protein-tyrosine phosphatase receptor type J; R-PTP-J; CD148
Fields
>>Adherens junction;>>Chemical carcinogenesis - reactive oxygen species
Human protein sequence Database
Q12913
Mouse protein sequence database
Q64455
Rat protein sequence database
Cellular localization
Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane . Cell junction. After T-cell stimulation, it is temporarily excluded from immunological synapses.
Tissue expression
Expressed in the promyelocytic cell line HL-60, the granulocyte-macrophage colony-stimulating factor-dependent leukemic cell line F-36P, and the IL3 and erythropoietin-dependent leukemic cell line F-36E. Expressed predominantly in epithelial cells and lymphocytes. Enhanced expression at high cell density.
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