Molecular weight (DA)
38kD
Immunogen
The antiserum was produced against synthesized peptide derived from human NT5C3. AA range:11-60
Specificity
NT5C3 Polyclonal Antibody detects endogenous levels of NT5C3 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
5'-nucleotidase, cytosolic IIIA(NT5C3A) Homo sapiens This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and pseudogenes of this gene are located on the long arm of chromosomes 3 and 4. [provided by RefSeq, Mar 2012],
Function
catalytic activity:A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate.,disease:Defects in NT5C3 are the cause of P5N deficiency [MIM:266120]; also called hemolytic anemia due to P5N deficiency or hemolytic anemia due to UMPH1 deficiency. P5N deficiency is an autosomal recessive condition causing hemolytic anemia characterized by marked basophilic stipplig and the accumulation of high concentrations of pyrimidine nucleotides within the erythrocyte. It is implicated in the anemia of lead poisoning and is possibly associated with learning difficulties.,function:Can act both as nucleotidase and as phosphotransferase.,induction:Isoform 2 is induced by interferon alpha in Raji cells in association with lupus inclusions.,similarity:Belongs to the pyrimidine 5'-nucleotidase family.,subunit:Monomer.,tissue specificity:Isoform 1 and isoform 3 are expressed in reticulocytes and lymphocytes.
Protein name
Cytosolic 5'-nucleotidase 3
Other name
NT5C3; P5N1; UMPH1; HSPC233; Cytosolic 5'-nucleotidase 3; Cytosolic 5'-nucleotidase III; cN-III; Pyrimidine 5'-nucleotidase 1; P5'N-1; P5N-1; PN-I; Uridine 5'-monophosphate hydrolase 1; p36
Fields
>>Pyrimidine metabolism;>>Metabolic pathways;>>Nucleotide metabolism
Human protein sequence Database
Q9H0P0
Mouse protein sequence database
Q9D020
Rat protein sequence database
Cellular localization
Cytoplasm .; [Isoform 2]: Endoplasmic reticulum.
Tissue expression
Isoforms 1, 3 and 4 are expressed in reticulocytes. Isoform 4 is hardly detectable in bone marrow and fetal liver.
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