Molecular weight (DA)
49kD
Immunogen
The antiserum was produced against synthesized peptide derived from human HRH3. AA range:291-340
Specificity
Histamine H3 Receptor Polyclonal Antibody detects endogenous levels of Histamine H3 Receptor protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and can regulate neurotransmitter release. This receptor can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system. [provided by RefSeq, Jul 2008],
Function
alternative products:Additional isoforms seem to exist,function:The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization.,miscellaneous:Does not bind to cimetidine and tripolidine. Shows modest affinity for thioperamide, imetit, N-alpha-methylhistamine and R(-)-alpha-methylhistamine. Isoform 4 is unable to bind to iodoproxyfan while isoforms 1 and 3 bind it with high affinity.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various is
Protein name
Histamine H3 receptor
Abbreviation
Histamine H3 Receptor
Other name
HRH3; GPCR97; Histamine H3 receptor; H3R; HH3R; G-protein coupled receptor 97
Fields
>>Neuroactive ligand-receptor interaction
Human protein sequence Database
Q9Y5N1
Mouse protein sequence database
P58406
Rat protein sequence database
Q9QYN8
Cellular localization
Cell membrane; Multi-pass membrane protein.
Tissue expression
Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level varies in a region-specific manner. Isoform 3 and isoform 7 are highly expressed in the thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra.
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