Molecular weight (DA)
69kD
Immunogen
The antiserum was produced against synthesized peptide derived from human DBH. AA range:401-450
Specificity
DBH Polyclonal Antibody detects endogenous levels of DBH protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008],
Function
catalytic activity:3,4-dihydroxyphenethylamine + ascorbate + O(2) = noradrenaline + dehydroascorbate + H(2)O.,cofactor:Binds 1 PQQ per subunit.,cofactor:Binds 2 copper ions per subunit.,disease:Defects in DBH are the cause of DBH deficiency [MIM:223360]; also called norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.,function:Conversion of dopamine to noradrenaline.,induction:Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids.,online information:Dopamine beta hydroxylase entry,pathway:Catecholamine biosynthesis; norepinephrine biosynthesis; norepinephrine from dopamine: step 1/1.,polymorphism:
Protein name
Dopamine beta-hydroxylase
Other name
DBH; Dopamine beta-hydroxylase; Dopamine beta-monooxygenase
Fields
>>Tyrosine metabolism;>>Metabolic pathways
Human protein sequence Database
P09172
Mouse protein sequence database
Q64237
Rat protein sequence database
Cellular localization
[Soluble dopamine beta-hydroxylase]: Cytoplasmic vesicle, secretory vesicle lumen . Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen . Secreted .; Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass type II membrane protein . Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane ; Single-pass type II membrane protein .
Tissue expression
Brain,Plasma,
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