Molecular weight (DA)
45kD
Immunogen
Synthesized peptide derived from human protein . at AA range: 80-160
Specificity
PGK2 Polyclonal Antibody detects endogenous levels of protein.
Formulation
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Dilution rate
WB 1:500-2000 ELISA 1:5000-20000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
phosphoglycerate kinase 2(PGK2) Homo sapiens This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010],
Function
catalytic activity:ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.,online information:Phosphoglycerate kinase entry,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.,similarity:Belongs to the phosphoglycerate kinase family.,subunit:Monomer.,tissue specificity:Testis specific.,
Protein name
Phosphoglycerate kinase 2 (EC 2.7.2.3) (Phosphoglycerate kinase, testis specific)
Fields
>>Glycolysis / Gluconeogenesis;>>Metabolic pathways;>>Carbon metabolism;>>Biosynthesis of amino acids;>>HIF-1 signaling pathway
Human protein sequence Database
P07205
Mouse protein sequence database
P09041
Rat protein sequence database
Cellular localization
Cytoplasm .
Tissue expression
Mainly found in round spermatids. Localized on the principle piece in the sperm (at protein level). Testis-specific. Expression significantly decreased in the testis of elderly men.