Molecular weight (DA)
48kD
Immunogen
Synthesized peptide derived from human protein . at AA range: 380-460
Specificity
SC65 Polyclonal Antibody detects endogenous levels of protein.
Formulation
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Dilution rate
WB 1:500-2000 ELISA 1:5000-20000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This nucleolar protein was first characterized because it was an autoantigen in cases on interstitial cystitis. The protein, with a predicted molecular weight of 50 kDa, appears to be localized in the particulate compartment of the interphase nucleolus, with a distribution distinct from that of nucleolar protein B23. During mitosis it is associated with chromosomes. [provided by RefSeq, Jul 2008],
Function
similarity:Belongs to the CRTAP/No55 family.,subcellular location:Localized uniformly throughout the granular component of the nucleolus and on the surface of chromosomes during mitosis.,
Gene Name
LEPREL4 NOL55 SC65
Protein name
Synaptonemal complex protein SC65 (Leprecan-like protein 4) (Nucleolar autoantigen No55)
Human protein sequence Database
Q92791
Mouse protein sequence database
Q8K2B0
Rat protein sequence database
Q64375
Cellular localization
Endoplasmic reticulum .
Tissue expression
Detected in fibroblasts (at protein level) (PubMed:23959653). Detected in spleen, prostate, testis, ovary, colon, pancreas, kidney, placenta and heart (PubMed:10952778).
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