Molecular weight (DA)
47kD
Immunogen
Synthesized peptide derived from part region of human protein
Specificity
ENOB Polyclonal Antibody detects endogenous levels of protein.
Formulation
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Dilution rate
WB 1:500-2000 ELISA 1:5000-20000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
enolase 3(ENO3) Homo sapiens This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010],
Function
catalytic activity:2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O.,cofactor:Magnesium. Required for catalysis and for stabilizing the dimer.,developmental stage:During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.,disease:Defects in ENO3 are the cause of muscle-specific enolase-beta deficiency (glycogenesis type XIII) [MIM:131370]. It is a glycogen storage myopathy which results in exercise-induced myalgias, generalized muscle weakness and fatigability. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen-beta particles are detected in patients.,function:Appears to have a function in striated muscle development and regeneration.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Protein name
Beta-enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (Enolase 3) (Muscle-specific enolase) (MSE) (Skeletal muscle enolase)
Fields
>>Glycolysis / Gluconeogenesis;>>Metabolic pathways;>>Carbon metabolism;>>Biosynthesis of amino acids;>>RNA degradation;>>HIF-1 signaling pathway
Human protein sequence Database
P13929
Mouse protein sequence database
P21550
Rat protein sequence database
P15429
Cellular localization
Cytoplasm. Localized to the Z line. Some colocalization with CKM at M-band (By similarity). .
Tissue expression
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
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