Molecular weight (DA)
110kD
Immunogen
Synthesized peptide derived from human protein . at AA range: 640-720
Specificity
ASAP2 Polyclonal Antibody detects endogenous levels of protein.
Formulation
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Dilution rate
WB 1:500-2000 ELISA 1:5000-20000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle
Function
domain:The conserved Arg-464 in the Arf-GAP domain probably becomes part of the active site of bound small GTPases and is necessary for GTP hydrolysis.,function:Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration.,induction:Up-regulated during monocyte maturation.,PTM:Phosphorylated on tyrosine residues by SRC and PTK2B.,similarity:Contains 1 Arf-GAP domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 2 ANK repeats.,subcellular location:Colocalizes with F-actin and ARF6 in phagocytic cups.,subunit:Binds PXN, ARF1, ARF5, ARF6, PTK2B and SRC.,tissue specificity:Detected in heart, brain, placenta, kidney, monocytes and pancreas.,
Gene Name
ASAP2 DDEF2 KIAA0400
Protein name
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 (Development and differentiation-enhancing factor 2) (Paxillin-associated protein with ARF GAP activity 3) (PAG3) (Pyk2 C-terminu
Fields
>>Endocytosis;>>Fc gamma R-mediated phagocytosis
Human protein sequence Database
O43150
Mouse protein sequence database
Q7SIG6
Rat protein sequence database
Cellular localization
Cytoplasm. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Colocalizes with F-actin and ARF6 in phagocytic cups.
Tissue expression
Detected in heart, brain, placenta, kidney, monocytes and pancreas.
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