Molecular weight (DA)
15kD
Immunogen
HA synthetic peptide conjugated to KLH.
Specificity
HA-tag Polyclonal Antibody detects HA-tagged recombinant proteins or HA-tagged proteins overexpressed in cells.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
Dilution rate
Western Blot: 1/1000 - 1/3000;. IP: 1:300 Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The HA tag (Y-P-Y-D-V-P-D-Y-A) is derived from an epitope (amino acids 98-106) of the influenza hemagglutinin (HA) protein. HA tag is used as a general epitope tag in expression vectors for many recombinant proteins. The presence of HA tag facilitates the detection, isolation and purification of recombinant fusion proteins.
Other name
HA epitope tag; hemagglutinin
Human protein sequence Database
Mouse protein sequence database
Rat protein sequence database