Immunogen
The antiserum was produced against synthesized peptide derived from human POFUT2. AA range:361-410
Specificity
O-FucT-2 Polyclonal Antibody detects endogenous levels of O-FucT-2 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008],
Function
catalytic activity:Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.,function:Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in thrombospondin type 1 repeats.,online information:GlycoGene database,online information:Peptide-O-fucosyltransferase 2,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyltransferase 68 family.,tissue specificity:Isoform A is expressed in fetal liver and peripheral blood lymphocytes. Isoform B is expressed in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, fetal brain, fetal liver and fetal kidney. Isoform C is expressed in brain, heart, spleen, liver, lung, stomach, testis, placenta, skin, thymus, pancreas, mammary gland, prostate, fetal brain, fetal liver and fetal heart.,
Protein name
GDP-fucose protein O-fucosyltransferase 2
Other name
POFUT2; C21orf80; FUT13; KIAA0958; GDP-fucose protein O-fucosyltransferase 2; Peptide-O-fucosyltransferase 2; O-FucT-2
Fields
>>Other types of O-glycan biosynthesis
Human protein sequence Database
Q9Y2G5
Mouse protein sequence database
Q8VHI3
Rat protein sequence database
Cellular localization
Endoplasmic reticulum . Golgi apparatus . Mainly located in the endoplasmic reticulum. .
Tissue expression
Isoform A is expressed in fetal liver and peripheral blood lymphocytes. Isoform B is expressed in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, fetal brain, fetal liver and fetal kidney. Isoform C is expressed in brain, heart, spleen, liver, lung, stomach, testis, placenta, skin, thymus, pancreas, mammary gland, prostate, fetal brain, fetal liver and fetal heart.
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