Immunogen
The antiserum was produced against synthesized peptide derived from human MRPS33. AA range:51-100
Specificity
MRP-S33 Polyclonal Antibody detects endogenous levels of MRP-S33 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that is one of the more highly conserved mitochondrial ribosomal proteins among mammals, Dro
Function
subunit:Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.,
Protein name
28S ribosomal protein S33 mitochondrial
Other name
MRPS33; CGI-139; PTD003; 28S ribosomal protein S33; mitochondrial; MRP-S33; S33mt
Human protein sequence Database
Q9Y291
Mouse protein sequence database
Q9D2R8
Rat protein sequence database
Cellular localization
Mitochondrion .
Tissue expression
Lung,Pituitary tumor,Pooled,
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