Molecular weight (DA)
23kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPL21. AA range:131-180
Specificity
MRP-L21 Polyclonal Antibody detects endogenous levels of MRP-L21 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Multiple transcript variants encoding different isoforms were identified through sequence analysis although some may be subject to nonsense-mediated decay (NMD). [provided by RefSeq, Jul 2008],
Function
similarity:Belongs to the ribosomal protein L21P family.,
Protein name
39S ribosomal protein L21 mitochondrial
Other name
MRPL21; 39S ribosomal protein L21; mitochondrial; L21mt; MRP-L21
Human protein sequence Database
Q7Z2W9
Mouse protein sequence database
Q9D1N9
Rat protein sequence database
Cellular localization
Mitochondrion .
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