Molecular weight (DA)
67kD
Immunogen
The antiserum was produced against synthesized peptide derived from human GRB10. AA range:33-82
Specificity
GRB10 Polyclonal Antibody detects endogenous levels of GRB10 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010],
Function
alternative products:Additional isoforms seem to exist,function:Plays a functional role in insulin and IGF-I signaling. May serve to positively link the insulin and IGF-I receptors to an uncharacterized mitogenic signaling pathway. Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor which is then inhibited. The interaction is mediated by the SH2 domain. Also binds activated platelet-derived growth factor receptor and epidermal growth factor receptor.,similarity:Belongs to the GRB7/10/14 family.,similarity:Contains 1 PH domain.,similarity:Contains 1 Ras-associating domain.,similarity:Contains 1 SH2 domain.,subunit:Interacts with GIGYF1/PERQ1 and GIGYF2/TNRC15.,tissue specificity:Highly expressed in skeletal muscle.,
Protein name
Growth factor receptor-bound protein 10
Other name
GRB10; GRBIR; KIAA0207; Growth factor receptor-bound protein 10; GRB10 adapter protein; Insulin receptor-binding protein Grb-IR
Fields
>>mTOR signaling pathway
Human protein sequence Database
Q13322
Mouse protein sequence database
Q60760
Rat protein sequence database
Cellular localization
Cytoplasm . When complexed with NEDD4 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment (By similarity). .
Tissue expression
Widely expressed in fetal and adult tissues, including fetal and postnatal liver, lung, kidney, skeletal muscle, heart, spleen, skin and brain.
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