Molecular weight (DA)
73kD
Immunogen
The antiserum was produced against synthesized peptide derived from human GCSc-gamma. AA range:266-315
Specificity
GCSc-γ Polyclonal Antibody detects endogenous levels of GCSc-γ protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010],
Function
catalytic activity:ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.,disease:Defects in GCLC are the cause of hemolytic anemia [MIM:230450].,enzyme regulation:Feedback inhibition by glutathione.,pathway:Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.,similarity:Belongs to the glutamate--cysteine ligase type 3 family.,subunit:Heterodimer of a catalytic heavy chain and a regulatory light chain.,
Protein name
Glutamate--cysteine ligase catalytic subunit
Other name
GCLC; GLCL; GLCLC; Glutamate--cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase
Fields
>>Cysteine and methionine metabolism;>>Glutathione metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors;>>Ferroptosis
Human protein sequence Database
P48506
Mouse protein sequence database
P97494
Rat protein sequence database
P19468
Cellular localization
cytosol,integral component of membrane,glutamate-cysteine ligase complex,
Tissue expression
Foreskin fibroblast,Hippocampus,Liver,Testis,Thalamus,
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