Molecular weight (DA)
50kD
Immunogen
The antiserum was produced against synthesized peptide derived from human CPA5. AA range:91-140
Specificity
CPA5 Polyclonal Antibody detects endogenous levels of CPA5 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Carboxypeptidases have functions ranging from digestion of food to selective biosynthesis of neuroendocrine peptides. Members of the A/B subfamily of carboxypeptidases, such as CPA5, contain an approximately 90-amino acid pro region that assists in the folding of the active carboxypeptidase domain. Cleavage of the pro region activates the enzyme (Wei et al., 2002 [PubMed 11836249]).[supplied by OMIM, Mar 2008],
Function
catalytic activity:Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.,cofactor:Binds 1 zinc ion per subunit.,similarity:Belongs to the peptidase M14 family.,tissue specificity:Expression is very low or not detectable.,
Protein name
Carboxypeptidase A5
Other name
CPA5; Carboxypeptidase A5
Human protein sequence Database
Q8WXQ8
Mouse protein sequence database
Q8R4H4
Rat protein sequence database
Cellular localization
Secreted .
Tissue expression
Expression is very low or not detectable.
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