Molecular weight (DA)
75kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MAP9. AA range:121-170
Specificity
MAP-9 Polyclonal Antibody detects endogenous levels of MAP-9 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
ASAP is a microtubule-associated protein required for spindle function, mitotic progression, and cytokinesis (Saffin et al., 2005 [PubMed 16049101]).[supplied by OMIM, Mar 2008],
Function
developmental stage:Constitutively expressed during the cell cycle.,function:Involved in organization of the bipolar mitotic spindle. Required for bipolar spindle assembly, mitosis progression and cytokinesis. May act by stabilizing interphase microtubules.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,subcellular location:Localizes to microtubules in interphase, associates with the mitotic spindle during mitosis, localizes to the central body during cytokinesis.,subunit:Binds to purified microtubules via its C-terminus.,
Protein name
Microtubule-associated protein 9
Other name
MAP9; ASAP; Microtubule-associated protein 9; Aster-associated protein
Human protein sequence Database
Q49MG5
Mouse protein sequence database
Q3TRR0
Rat protein sequence database
Cellular localization
Cytoplasm . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, spindle . Localizes to microtubules in interphase, associates with the mitotic spindle during mitosis, localizes to the central body during cytokinesis.
Tissue expression
Amygdala,Bone marrow,Brain,
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