Molecular weight (DA)
55kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 21A2. AA range:151-200
Specificity
CYP21A2 Polyclonal Antibody detects endogenous levels of CYP21A2 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
cytochrome P450 family 21 subfamily A member 2(CYP21A2) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia. A related pseudogene is located near this gene; gene conversion events involving the functional gene and the pseudogene are thought to account for many cases of steroid 21-hydroxylase deficiency. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function
catalytic activity:A steroid + AH(2) + O(2) = a 21-hydroxysteroid + A + H(2)O.,cofactor:Heme group.,disease:Defects in CYP21A2 are the cause of adrenal hyperplasia type 3 (AH3) [MIM:201910]. AH3 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late onset (NC or LOAH), and 'cryptic' (asymptomatic).,domain:The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal
Protein name
Steroid 21-hydroxylase
Other name
CYP21A2; CYP21; CYP21B; Steroid 21-hydroxylase; 21-OHase; Cytochrome P-450c21; Cytochrome P450 21; Cytochrome P450 XXI; Cytochrome P450-C21; Cytochrome P450-C21B
Fields
>>Steroid hormone biosynthesis;>>Metabolic pathways;>>Aldosterone synthesis and secretion;>>Cortisol synthesis and secretion;>>Cushing syndrome
Human protein sequence Database
P08686
Mouse protein sequence database
P03940
Rat protein sequence database
Cellular localization
Endoplasmic reticulum membrane; Peripheral membrane protein . Microsome membrane ; Peripheral membrane protein .
Tissue expression
Adrenal gland,PCR rescued clones,Peripheral blood,
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